Western Blot:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Purification:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Incubation:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Transfection:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Microscopy:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Expressing:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Immunoprecipitation:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Binding Assay:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Lysis:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Inhibition:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Transformation Assay:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Migration:Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: Plasmids The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
Article Title: The ubiquitin specific protease 7 stabilizes HPV16E7 to promote HPV-mediated carcinogenesis
Article Snippet: The pDONR201: Flag-tagged USP7 (RRID: Addgene_104959) was kindly provided by Prof. HUEN, Michael Shing Yan; pGEX-2T: HA-HPV16E7 (RRID: Addgene_13634), E7 NT (amino acid 1–52) and E7 CT (amino acid 44–98); and pCMV: HA-HPV16E7 (RRID: Addgene_13686), HA-Rb (RRID: Addgene_58905) and HA-ubiquitin (RRID: Addgene_131258) were kind gifts from Dr. Lawrence Banks.. All plasmids were transformed into Escherichia coli strain DH5-α for amplification.All plasmids were transformed into Escherichia coli strain DH5-α for amplification.
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